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Brilliant Blue BiosciencesNanobiotechnology Research
ACS Nano2024Drug Delivery

Twelve-channel quantum-dot multiplexing for point-of-care biomarker panels

A. Okonjo, T. LindDOI 10.1021/acsnano.2024.00000

Summary

Cadmium-free quantum dots with narrow emission profiles allow twelve biomarkers to be read from a single chip in under ten minutes.

Multiplexed immunoassays usually trade channel count against cross-talk.

By engineering cadmium-free quantum dots with full-width-half-maximum below 28 nm, we separated twelve emission channels while keeping spectral overlap low enough to skip matrix deconvolution.

The panel resolved all twelve targets in a single nine-minute run at femtomolar sensitivity, and the reader tolerated 15 to 40 °C ambient — a prerequisite for field deployment.

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Twelve-channel quantum-dot multiplexing for point-of-care biomarker panels | Brilliant Blue Biosciences