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Resources & Tools

Video Tutorials

44 expert-led videos — step-by-step transfection demos, live-cell imaging, LNP deep dives and characterisation walkthroughs. Play them here or open each one on YouTube.

Transfection fundamentals

What transfection is, how cargo crosses the membrane and how to work with the cell lines everyone starts with.

Transfection protocols & reagents

Bench demonstrations of lipid, polymer and physical transfection — from complex formation to expression.

Lipofectamine® 3000 - Transfection Reagent Kit

Thermo Fisher Scientific

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Lipofectamine® MessengerMAX™ mRNA Transfection Reagent

Thermo Fisher Scientific

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Transfection: Time-Lapse video of transfected HeLa cells

Curiosis Inc.

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Xfect™ Single Shots - State-of-the-art transfection technology

that'sGOODscience!

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siRNA & CRISPR delivery

Delivering small RNAs and Cas9 ribonucleoproteins for knockdown and genome editing.

Visualize Your CRISPR Transfection with Cas9-GFP Protein

MilliporeSigma

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Plasmid DNA preparation

Extracting and purifying the plasmid DNA that goes into every transfection.

Lipid nanoparticles & mRNA

How LNPs are built, manufactured and escape the endosome to deliver mRNA.

Manufacturing RNA lipid nanoparticles

Precision NanoSystems / Cytiva

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Endosomal Escape of Lipid Nanoparticles: Novel Insights Using Reflectivity Techniques

Precision NanoSystems / Cytiva

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Enhancing Endosomal Escape and Applications to Extrahepatic mRNA Delivery

Precision NanoSystems / Cytiva

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Gene therapy & nanoparticle delivery

Viral transduction, nanoparticle gene delivery and the principles of gene therapy.

Lentivirus Transduction Protocol: Infecting your target cells

abm

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Characterisation & analysis

DLS, flow cytometry, transfection efficiency and cell viability — the assays that validate delivery.

A basic introduction to Dynamic Light Scattering (DLS) for particle size analysis

Malvern Panalytical

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How to Start with the Attune NxT Flow Cytometer

Thermo Fisher Scientific

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MTT Assay for Cell Viability

The Ahmed Lab: Northwestern Neurosurgery

Watch on YouTube
Video Tutorials | Brilliant Blue Biosciences