Papers, explained in our own words

1. Lipoplex Nanoparticle-Based RNA Delivery to 3D Melanoma Spheroids

Rudolf R, Cesetti T, Keller F, Hafner M

Lipoplex nanoparticle-based vaccination technologies have shown tremendous efficiency for in vivo transfection during the COVID-19 pandemic and also hold promise for treating melanoma. While pharmacodynamics and pharmacokinetics need careful in vivo and clinical testing, other parameters of drug performance can be…

• CCD-1137Sk
• Fibroblast
• HaCaT
• Keratinocyte
• LPX
• Melanoma cell
• SK-MEL-28
• Spheroid
• Transfection

2. The synthetic biology frontier: engineered oncolytic viruses and viral vectors for next-generation cancer therapy and gene delivery

Alsharedeh Rh, Aljabali Aaa, Obeid Ma, Alqudah A, Gammoh O, Qnais E, Et Al.

Viruses are no longer used merely as naturally occurring tools for gene transfer or as blunt cytolytic agents. Researchers are rewriting their genomes, capsids, tropisms, regulatory elements, and payloads to create therapeutic systems that sense disease, execute conditional programs, and remodel pathological tissue…

3. Nanotechnology-Based Drug Delivery for Parkinson's Disease: A Review On Strategies, Efficacy and Translational Hurdles

Hari M, Subramony A, Dwivedi D, Krishnan Rs, Damodaran A, Nair Sc

Treatment for Parkinson's disease (PD) faces two significant challenges: first, the blood-brain barrier (BBB) and second, a therapeutic strategy that alleviates symptoms instead of stopping the disease progression. The BBB prevents over 98% of small-molecule drugs, leaving neuroprotective options mostly inaccessible…

• Drug delivery
• Nanoparticles
• Nanotechnology
• Parkinson’s disease
• Targeted therapy

4. Inhalable Nanomedicines for Chronic Obstructive Pulmonary Disease: Recent Developments and Translational Barriers

Ainurofiq A, Rosalin Ea, Istiyono Ms, Maharani Na, Fauzy R, Sugiharti Sn, Et Al.

Chronic Obstructive Pulmonary Disease (COPD) is a condition that poses major challenges in therapeutic management, primarily due to the limitations of conventional inhalation methods in achieving optimal drug deposition and maintaining therapeutic effects over a long period. Inhalable nanomedicine has emerged as an…

• COPD
• Drug delivery
• Inhaled nanomedicine
• Nanoparticles
• Pulmonary therapy

5. Interpretable AI-guided discovery of degradable ionizable lipids with enhanced hepatic mRNA delivery in vivo

Zhang Z, Ren Y, Kong D, Feng J, Hu K, Xu X, Et Al.

Lipid nanoparticles (LNPs) enable RNA medicines, yet ionizable-lipid discovery remains largely empirical, particularly when in vitro activity must be translated into functional hepatic delivery in vivo. Here we establish an interpretable discovery framework that combines modular degradable lipid chemistry…

6. Overcoming oxaliplatin resistance and toxicity in colorectal cancer: the emerging role of lipid-based nanocarriers

Chauhan A, Kumar M, Awasthi A

To provide a comprehensive overview of lipid-based nanocarriers for oxaliplatin (OXA) delivery in colorectal cancer (CRC), with emphasis on their potential to improve pharmacokinetics, enhance tumour accumulation, enable controlled drug release, and overcome OXA resistance. Relevant literature on OXA, CRC…

• OXA resistance
• colorectal cancer
• lipid based nanocarriers
• targeted drug delivery
• tumor microenvironment

7. Nanomaterials for endometriosis: a multimodal strategy integrating targeted delivery, immunomodulation, and physical ablation

Inam M, Hayat M, Latif F, Ahmed B, Osman A

Endometriosis is a chronic inflammatory condition for which existing treatments, mainly hormonal therapy and surgical intervention, remain insufficient. These approaches are often limited by high recurrence rates, significant side effects, and an inability to effectively target underlying disease mechanisms. This…

• endometriosis
• immunomodulation
• magnetic hyperthermia
• nanocarrier
• nanomedicine
• photothermal therapy

8. PLGA Based "Lipid-Polymer Hybrid Nanoparticles": A Convergent Platform of Active and Targeted Drug Delivery

Patro R, Patra Cn, Mallick S

A promising class of nano-carriers known as "Lipid-Polymer Hybrid Nanoparticles" (LPHNPs) combines the biocompatibility and functionality of lipid-based systems with the structural advantages of polymeric nanoparticles. This review summarizes structural classifications and preparation methods of PLGA-based…

• Lactic-glycolic acid polymer
• Targeted drug delivery
• “Nano Lipid-Polymer Hybrid”

9. Chemical Design and Therapeutic Efficacy of HBsAg-Based Nanovaccines for Hepatitis B Virus Clearance: A Preclinical and Clinical Systematic Review

Nequi Marnani H, Zadeh Mehrizi T, Eshrati B, Shafiee Ardestani M, Ajdary N, Vodjgani M, Et Al.

More than 240 million people worldwide are chronically infected with hepatitis B. Although current antiviral therapies effectively inhibit viral replication, they rarely result in a functional cure. The development of hepatitis B surface antigen (HBsAg)-based nanovaccines has emerged as a potential therapeutic…

• Chronic
• Hepatitis B
• Hepatitis B surface antigens
• Nanovaccines
• Therapeutic

10. Spray-Dried mRNA-LNP Powder Enabling Multi-Route Delivery With 3-Year Refrigerated Stability

Sarode A, Patel P, Derosa F, Karve S, Gervier R

The clinical translation of mRNA-lipid nanoparticle (mRNA-LNP) therapeutics is constrained by limited stability of liquid formulations, necessitating ultra-cold storage and restricting global accessibility. Here, we report a spray-dried dry powder platform for mRNA-LNPs that overcomes these limitations while enabling…

• dry powder
• lipid nanoparticles
• mRNA therapeutics
• mucosal
• spray drying
• thermostability

11. Functionalized nanocarrier-based intranasal drug delivery of rivastigmine tartrate for enhanced brain exposure: a pharmacokinetic and biodistribution evaluation

Shetty S, Suvarna V

To develop and evaluate a polyethylene glycol (PEG)-functionalized multi-walled carbon nanotube (MWCNT)-based intranasal delivery system for rivastigmine tartrate (RT) to improve systemic exposure and brain delivery for Alzheimer's disease (AD). RT-loaded PEGylated MWCNTs were prepared, optimized using a 3 2…

• Alzheimer’s disease
• Rivastigmine tartrate
• brain bioavailability
• intranasal delivery
• multi-walled carbon nanotubes
• nose-to-brain targeting

12. Bifunctional EGFR/CD44-Targeted Diabody-Decorated Doxorubicin-Loaded Solid Lipid Nanoparticles for Targeted Therapy in Triple-Negative Breast Cancer

Arastehfar M, Soleimani Badie M, Derakhshandeh K

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that lacks targeted therapies, making its treatment highly challenging. This study aimed to design a novel dual-targeted drug delivery system to improve therapeutic efficacy and reduce the side effects of conventional chemotherapy. Solid…

• Breast cancer
• Diabody
• Doxorubicin
• Solid lipid nanoparticle

13. Protocol for the production of high-yielded research-grade AAV vectors in transiently transfected HEK293T cells

Duke L, Kantor B

Adeno-associated vector is a platform of choice for delivering genome-editing toolkits, due to its transient and robust expression. Here, we present a protocol for viral production for in vivo use. We describe steps for transfecting producer cells, harvesting virus, and purifying/concentrating viral particles. We…

• CRISPR
• Cell Biology
• Cell culture
• Cell-based Assays
• Genetics
• Genomics
• Molecular Biology
• Protein Biochemistry

14. Intranasal nanocarrier-based drug delivery in multiple sclerosis: therapeutic cargo, CNS targeting and translational challenges

Parseghian L, Sanadgol N, Negah Ss, Fahanik-Babaei J, Rafati H

Efficiently delivering therapeutics to the central nervous system (CNS) remains a major challenge in multiple sclerosis (MS), particularly for strategies targeting CNS-resident pathology, neuroprotection, or remyelination. This review provides a delivery- and formulation-centered comparative analysis of CNS drug…

• Blood–brain barrier
• CNS targeting
• Intranasal delivery
• Multiple sclerosis
• Nanocarriers
• Nanomedicine
• Nanotechnology
• Nose-to-brain transport
• Surface-functionalized nanoparticles

15. Effect of Exosome-loaded hsa-miR-203a-3p on Triple Negative Breast Cancer Cell Viability, Migration, and Gene Targets: In Silico and In Vitro Study

Ardiansyah Sa, Noficandra H, Satriyo Pb, Nugrahaningsih Daa, Haryana Sm

Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype lacking targeted therapy options. MicroRNA-based therapy, particularly hsa-miR-203a-3p, has shown tumor-suppressive potential; however, an effective delivery system is required to enhance its stability and cellular uptake. Exosomes represent…

• Exosome
• In silico
• Triple negative breast cancer
• hsa-miR-203a-3p

16. Human Serum Albumin as a Smart Nanocarrier for [RuNO(CQ)(POA)Cl] Isomeric Complexes: Structural, Photodynamic, and Antitumor Activity Insights

Pang Y, Gong W, Liu C, Ma D, Liu H, Wu Y, Et Al.

Human serum albumin (HSA) is a natural and non-immunogenic nanocarrier with multifunctional binding pocket for various small bioactive molecules. Hereby, four RuNO(CQ)(POA)X (Ru 1-4) complexes with different configurations were synthesized, where CQ is 5-Cl-7-I-8-hydroxyquinoline, POA is 2-Pyrazinecarboxylic acid…

• Cytotoxicity
• HSA
• NO
• Nitroyl ruthenium complex
• Photodynamic

17. Modular Peptide Coatings Regulate the Multistep Biological Performance of Lipid Nanoparticles for Enhanced mRNA Delivery

Tang C, Ye Y, Min J, Han M, Jiao X, Su R, Et Al.

Lipid nanoparticles (LNPs) are a leading platform for mRNA delivery, yet improving their performance often requires complex chemical modification or reformulation of core components. Here, we report a modular peptide-coating strategy for post-formulation engineering of LNP interfaces through physical assembly. A…

• biological barriers
• lipid nanoparticle
• mRNA delivery
• peptide
• surface engineering
• tumor immunotherapy

18. Theranostic lipid nanocarriers for precision diagnosis and targeted therapy in pancreatic ductal adenocarcinoma

Nagdev S, Tantray J, Yadav Mk, Patel A, Sharma S, Singh Ak, Et Al.

Pancreatic cancer is among the most prevalent ones, and its diagnosis typically occurs in the latter stages. Traditional chemotherapy is usually limited by low tumour penetration, systemic toxicity, and drug resistance. The new method of drug delivery utilizing nanotechnology has proven an effective strategy for…

• Clinical translation
• Nanocarriers
• Nanomedicine approval
• Pancreatic cancer
• Regulatory framework

19. Insights into Composition-Induced Structural Shifts in Lipid Nanoparticles

Wei Rj, Fuladi S, Grzetic Dj, Misra M, Hughes Tf, Giesen Dj, Et Al.

Lipid nanoparticles (LNPs) are a leading platform for nucleic acid delivery, yet current formulations typically suffer from rapid in vivo clearance and limited extrahepatic delivery. Recent experiments demonstrate that increasing the helper lipid content (e.g., to 40% 1,2-distearoyl-sn-glycero-3-phosphocholine DSPC)…

20. Nanotechnology-enabled drug delivery for retinoblastoma: formulation strategies, ocular targeting and translational perspectives

Malak Q, Mazumder R, Rani A, Dogra A

Retinoblastoma is an aggressive intraocular tumor in children, with a major underlying pathogenesis driven by mutations in the RB1 gene. Despite advances in management, current treatments, including systemic chemotherapy, focal therapies, radiotherapy, and enucleation, are limited by systemic toxicity, ocular…

• Retinoblastoma
• immunotherapy
• nanoparticles
• ocular drug delivery
• targeted therapies

21. Lipid nanoparticles adjuvanted with poly(I:C) enhance respiratory mucosal immunity induced by a protein subunit vaccine

Da Costa Rodrigues T, Kola Ns, Patel D, Ibrahim A, Ma'aruf Sy, Haddadi A, Et Al.

Subunit vaccines are typically given through injections, which limits mucosal immunity. Most adjuvants do not stimulate mucosal responses but using lipid nanoparticles (LNPs) encapsulating polyinosinic-polycytidylic acid (poly(I:C)), a toll-like receptor 3 agonist, could enhance the immune response in the lungs. We…

• Adjuvant
• IgA
• Lipid nanoparticles
• Protein subunit vaccine
• Resident memory T cells
• Respiratory mucosal immunity
• poly(I:C)

22. Harnessing exosomes for breast cancer: bridging conceptual promise to tangible translational application

Kaur S, Harish V, Bashir B, Kolekar Ka, Singh Sk

Exosomes have been described as emerging nanocarriers that have potential for the targeted delivery of therapeutics. They offer many advantages over traditional drug delivery systems, such as their intrinsic biocompatibility, low immunogenicity, and prolonged circulation, as well as crossing biological barriers. This…

• Breast cancer
• Clinical translation
• Exosomes
• Multidrug resistance
• Nanotechnology
• Tumour microenvironment

23. Dual drug delivery via integrin-targeted nanoparticles enhances therapeutic efficiency in BRAFV600E colon cancer

Sen R, Alnatour M, Janardhanam Lsl, Anjum Mm, Naguib Yw, Geary Sm, Et Al.

Colorectal cancer (CRC) patients possessing the BRAF V600E mutation often fail to respond to current first line therapies and are associated with poor prognosis, despite the advent of orally delivered small molecule inhibitors (SMIs) targeting the RAS-RAF-MEK-ERK signaling pathway. Thus, further improvements are…

• Colon cancer
• Dual targeting
• Small molecule inhibitors (SMIs)
• Therapeutic efficacy

24. Elucidating the Structural Features of a Class of PAMAM-Acyl Synthetic Lipid Nanoparticles

Hall Cb, Tan L, Okai Dna, Dobler S, Adigun R, Okuwobi Cb, Et Al.

Herein, we characterize a class of PAMAM/acyl synthetic lipids and their self-assembled nanostructures. We explicitly determined the impact of hydrocarbon length, saturation, and synthetic lipid volume on the properties of the self-assembled, single-component nanostructure. The synthesis of the PAMAM-acyl lipid…

• drug delivery
• lipid nanoparticles
• lipids
• synthetic lipids