Methods, data and protocols — in full detail
Everything a formulation or delivery scientist needs to evaluate our platform: how we make and measure carriers, the protocols we use and the literature behind them.
How we make and measure a carrier
- Step 1
Design
Ionisable lipid, helper lipid, cholesterol and PEG-lipid ratios chosen by design of experiments, not by habit.
- Step 2
Formulate
Microfluidic mixing at controlled total flow rate and flow-rate ratio, from 5 mL screening batches upwards.
- Step 3
Characterise
Size and PDI by DLS, zeta potential, encapsulation efficiency, cryo-TEM morphology and apparent pKa by TNS assay.
- Step 4
Test
Transfection, viability and uptake in relevant cell panels, then biodistribution with matched imaging labels.
See the mechanism animated on the technology page.
BrilliantCore™ at a glance
- Particle size range
- 12–80 nm
- Polydispersity index
- < 0.09
- Encapsulation efficiency
- 96.4%
- Batch scale
- 5 mL → 50 L
- Storage stability
- 30 days @ 4 °C
- Endotoxin
- < 0.25 EU/mL
Run it in your own lab
- Transfection guide →Reagent choice, optimisation and troubleshooting by cell type.
- Assay Library →25 characterisation and biological assays with purpose and readouts.
- Video tutorials →Expert-led walkthroughs of LNP formulation and analysis.
- Glossary →Definitions for gene delivery and nanomedicine terms.
Latest literature reviews
- Advances in lipids design for LNP-mediated DNA and RNA delivery
Advances in Colloid and Interface Science
- Recent Developments in Lipid Nanoparticle-Mediated Delivery of Biotherapeutics and Gene Therapy Across the Blood-Brain Barrier
BioDrugs
- Recent Advances in Lipid Nanoparticles: Nucleic Acid Therapeutics and Targeting Strategies
Small
- Rational design of lipid nanoparticles for enabling gene therapies
Molecular Therapy Methods & Clinical Development
White papers & application notes
Let's engineer the next delivery breakthrough together
We co-develop nanocarrier and biosensing programmes with pharma, biotech and academic groups — from target selection through GMP supply.
Prefer to talk it through? Book a 30-minute discovery call