Orthogonal ensemble and single-particle characterisation of antisense oligonucleotide lipid nanoparticles
Summary
Antisense oligonucleotide (ASO) lipid nanoparticles (LNPs) remain underexplored relative to siRNA and mRNA LNPs. Using an orthogonal workflow (DLS, NTA, AF4), we show that ionisable lipid identity and microfluidic flow rate govern key LNP attributes. Replacing SM-102 with MC3 increased size and reduced ASO recovery, while lower flow rates increased LNP size and improved recovery, highlighting formulation-property relationships for ASO LNP optimisation.
Antisense oligonucleotide (ASO) lipid nanoparticles (LNPs) remain underexplored relative to siRNA and mRNA LNPs. Using an orthogonal workflow (DLS, NTA, AF4), we show that ionisable lipid identity and microfluidic flow rate govern key LNP attributes. Replacing SM-102 with MC3 increased size and reduced ASO recovery, while lower flow rates increased LNP size and improved recovery, highlighting formulation-property relationships for ASO LNP optimisation.
Abstract from PubMed Central (PMID 43017426, PMC13752843). This entry was added automatically by our daily literature monitor because it matches the topics we follow; read the full paper at the original source.
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