Protocols and procedure

Step-by-step transfection protocols, by method

Choose a method family to see each reagent candidate, the manufacturer's dosing basis, the step-by-step procedure and the references behind it.

Reagents

Types of transfection methods

Lipid-based

Lipofectamine, X-tremeGENE, Effectene, FuGENE, ViaFect

Form complexes with nucleic acids and fuse with cell membranes. Preferred for high efficiency in standard lines such as HEK293 and CHO-K1; generally easy to use and less toxic.

9 protocols

Lipofectamine 2000
Lipofectamine 3000
Lipofectamine RNAiMAX
Lipofectamine MessengerMAX
X-tremeGENE HP DNA
X-tremeGENE 9 DNA
Effectene
FuGENE HD
ViaFect

Polymer-based

PEI, TurboFect, jetPRIME, jetOPTIMUS, DreamFect

Cationic polymers condense nucleic acids. Often used for difficult-to-transfect cells or when cost matters; typically cost-effective, stable and frequently serum-compatible.

7 protocols

PEI 25 kDa (branched)
TurboFect
jetPRIME
jetOPTIMUS
FuGENE 6 / FuGENE HD (polymer components)
DreamFect Gold
PEIpro

Physical methods

Electroporation, nucleofection, microinjection, magnetofection

Transient membrane pores let cargo in directly. Often the only viable option for primary immune cells and CAR-T engineering, but needs equipment and careful recovery.

5 protocols

Lonza Nucleofector (4D / SE cell line kits)
Neon Transfection System
MaxCyte flow electroporation
Microinjection
Magnetofection

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