All-in-One Prime Editing System for Efficient Generation and Isolation of Single Edited hiPSC Clones

Prime editing has emerged as the preferred gene-editing technique due to its versatility and precision in introducing genetic modifications such as single-base pair substitutions, insertions, and deletions without the need for double-strand breaks (DSBs) and a donor template. Thus, substantially reduces the likelihood of off-target effects that are generally produced when using the traditional CRISPR-Cas9 system. Here, we describe an in-depth protocol for the generation and isolation of prime-edited single human induced pluripotent stem cell (hiPSC) clones. In this example, we transfect a commercial hiPSC line with an all-in-one prime editor plasmid to install a pathogenic PRPH2 mutation using the PE3 approach.

Abstract from PubMed (PMID 43015546). This entry was added automatically by our daily literature monitor because it matches the topics we follow; read the full paper at the original source.