Large Insertion Using Template-Jumping Prime Editing
Summary
Prime editors (PEs) are advanced gene-editing tools capable of a wide spectrum of genomic modifications, offering significant potential for gene therapy. The template-jumping prime editing (TJ-PE) system employs template-jumping prime editing guide RNAs (TJ-pegRNAs) with a dual primer binding site (PBS) sequence. One PBS anneals to the DNA flap generated by the TJ-pegRNA, while the other binds to the DNA flap generated by the nicking sgRNA, enabling the accurate insertion of large DNA fragments.
Prime editors (PEs) are advanced gene-editing tools capable of a wide spectrum of genomic modifications, offering significant potential for gene therapy. The template-jumping prime editing (TJ-PE) system employs template-jumping prime editing guide RNAs (TJ-pegRNAs) with a dual primer binding site (PBS) sequence. One PBS anneals to the DNA flap generated by the TJ-pegRNA, while the other binds to the DNA flap generated by the nicking sgRNA, enabling the accurate insertion of large DNA fragments.
Abstract from PubMed (PMID 43015547). This entry was added automatically by our daily literature monitor because it matches the topics we follow; read the full paper at the original source.
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