Plant Prime Editing: Design, Assembly, and Application
Summary
Prime Editing (PE) is the latest technological advancement in genome editing. With the capabilities of altering the genome with precision and efficiency, PE offers great advantages over conventional CRISPR-Cas9 as well as base editors to achieve all 12 possible base changes and small indels. However, the complicated design and assembly of prime editing constructs is a limiting step in the wider applicability of the technology.
Prime Editing (PE) is the latest technological advancement in genome editing. With the capabilities of altering the genome with precision and efficiency, PE offers great advantages over conventional CRISPR-Cas9 as well as base editors to achieve all 12 possible base changes and small indels. However, the complicated design and assembly of prime editing constructs is a limiting step in the wider applicability of the technology. In this chapter, we set forth step-by-step protocols aimed at helping the scientific community in designing their constructs as well as assembling them to target up to four genes. With the availability of PE plasmids and design/assembly protocols, PE in plants can be carried out by more labs across the globe to recognize the great potential of PE in their research.
Abstract from PubMed (PMID 43015554). This entry was added automatically by our daily literature monitor because it matches the topics we follow; read the full paper at the original source.
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