Methods in molecular biology (Clifton, N.J.)2027ResearchNon-viral Gene Delivery
1. All-in-One Prime Editing System for Efficient Generation and Isolation of Single Edited hiPSC Clones
Theodore K, Tahir S, Costa Bld, Quinn Pmj
Prime editing has emerged as the preferred gene-editing technique due to its versatility and precision in introducing genetic modifications such as single-base pair substitutions, insertions, and deletions without the need for double-strand breaks (DSBs) and a donor template. Thus, substantially reduces the…
- Cell line engineering
- Human induced pluripotent stem cells (hiPSCs)
- Inherited retinal disease (IRD)
- Peripherin-2 (PRPH2)
- Prime editing