Nature Reviews Drug Discovery2005ReviewNon-viral Gene Delivery
Pack, D. W.; Hoffman, A. S.; Pun, S.; Stayton, P. S
The lack of safe and efficient gene-delivery methods remains a limiting obstacle to human gene therapy. Synthetic vectors are safer than recombinant viruses but generally lack sufficient efficacy, so polymers must be rationally designed to overcome extracellular and intracellular delivery barriers. PEI: Only 15–20%…
Read the article →Synthesis of Poly(β-amino esters) Optimized for Highly Effective Gene Delivery.2003ResearchNon-viral Gene Delivery
Akinc, A., Anderson, D. G., Lynn, D. M., & Langer, R. (2003).
Nonviral polymeric gene delivery vectors need systematic optimisation of nonstructural factors—polymer molecular weight, chain end-group, and polymer/DNA ratio—that strongly influence transfection, cytotoxicity, and DNA complexation. These factors had not been comprehensively studied together for poly(β-amino…
Read the article →Gene Therapy2001ResearchNon-viral Gene Delivery
Primary lymphoid cells, especially human peripheral blood mononuclear cells (PBMC), are very difficult to transfect with viral and nonviral vectors. There is a need for efficient nonviral, receptor-mediated gene delivery to PBMC for cell-based therapies and for studying gene function in primary lymphocytes. Naive vs…
Read the article →2001ReviewNon-viral Gene Delivery
Kircheis, R.; Wightman, L.; Wagner, E
PEI is one of the most effective non-viral gene delivery polymers, but its efficacy, toxicity, and in vivo behaviour depend strongly on molecular weight, branching, particle size, surface charge, and modifications. This review addresses how PEI/DNA complexes can be modified with targeting ligands, shielding agents…
Read the article →1999ReviewNon-viral Gene Delivery
Godbey, W. T.; Wu, K. K.; Mikos, A. G
Gene therapy still lacks safe and efficient delivery vectors. PEI has emerged as a promising non-viral polycation because it condenses DNA and mediates endosomal escape, but its transfection mechanism, targeting potential, and toxicity require clearer understanding. This review summarises PEI chemistry, PEI/DNA…
Read the article →ResearchNon-viral Gene Delivery
Peng Zhang, Qinan Xu, Xinfang Li, Youxiang Wang
Non-viral gene vectors must overcome multiple extracellular and intracellular barriers: stability in blood, efficient cellular uptake, endosomal escape, and intracellular gene release. Stimuli-responsive carriers can address some barriers, but a carrier capable of overcoming multiple barriers simultaneously is still…
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