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Research Review

Showing 1–1 of 1 articles · Keyword: Protein Biochemistry Clear keyword

STAR protocols2026ResearchNon-viral Gene Delivery

1. Protocol for the production of high-yielded research-grade AAV vectors in transiently transfected HEK293T cells

Duke L, Kantor B

Adeno-associated vector is a platform of choice for delivering genome-editing toolkits, due to its transient and robust expression. Here, we present a protocol for viral production for in vivo use. We describe steps for transfecting producer cells, harvesting virus, and purifying/concentrating viral particles. We…

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