Iranian journal of medical sciences2026ResearchNon-viral Gene DeliveryDrug Delivery
Raji S, Soltaninejad H, Hosseinkhani S, Hamidieh Aa
Leukocyte adhesion deficiency (LAD) is a genetic immunodeficiency that severely impairs the leukocyte adhesion cascade and migration to the sites of infection and inflammation. As a result, the main consequence is recurrent and severe infections with bacteria and fungi, which can lead to death. The most common…
Read the article →Current gene therapy2026ResearchNon-viral Gene DeliveryDrug Delivery
Rajeshwar V, Kumar Kr, Kumar Gr, Roja P, Mohan Vk, Aparna Tn
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated protein 9 (Cas9) system has revolutionized genome editing through its precision, simplicity, and programmability. The clinical translation of CRISPR/Cas9 Ribonucleoproteins (RNPs) remains constrained by major delivery challenges…
Read the article →Biomaterials2026ResearchNon-viral Gene DeliveryDrug Delivery
Chen Y, Li P, Xie Y, Wujieti B, Cui W, Zhang H, Et Al.
Lung cancer is the leading cause of cancer-related deaths worldwide. Among them, non-small cell lung cancer (NSCLC) accounts for approximately 85% of all cases, while the mutation of KRAS oncogene plays a crucial role in its formation and metastasis. Here, we report a transcription activator-like effector (TALE) base…
Read the article →Applied microbiology and biotechnology2026ResearchNon-viral Gene DeliveryDrug Delivery
Farahani Ms, Shamsara M, Moazzeni Sm, Khalili S, Johnson B, Darzi L, Et Al.
Among the emerging cancer therapies, immunotherapy using T cell engineering stands out as one of the most promising approaches. While the clustered regularly interspaced palindromic repeats (CRISPR/Cas) system is widely recognized as one of the most common gene-editing system tools, the main challenge for in vivo…
Read the article →Advanced science (Weinheim, Baden-Wurttemberg, Germany)2026ResearchNon-viral Gene DeliveryDrug Delivery
He L, Yu D, Xie F, Hou K, Li Z, Dai M, Et Al.
Despite the remarkable clinical success of immunotherapy, its efficacy against hepatocellular carcinoma (HCC) remains severely limited, highlighting an urgent need for novel targets and strategies to overcome immune evasion. Here, we aimed to identify key regulators of HCC immunity via unbiased functional screening…
Read the article →Nanomaterials2021ResearchNon-viral Gene Delivery
Tresa Lopez-Royo, Victor Sebastian, Laura Moreno-Martinez, Laura Uson, Cristina Yus, Teresa Alejo, Pilar Zaragoza, Rosario Osta, Manuel Arruebo, Raquel Manzano
CRISPR/Cas gene-editing components are often encoded on large plasmids (9–19 kb), which are difficult to encapsulate and transfect. Most PLGA nanoparticle (NP) gene-delivery studies use small nucleic acids (siRNA) or small plasmids (<6 kb), and the structural integrity and functional performance of large plasmids in…
Read the article →Biomaterials Science2019ResearchNon-viral Gene Delivery
Brynn R. Olden, Emmeline Cheng, Yilong Cheng, Suzie H. Pun
T cells are an important target for ex vivo gene delivery and editing, but most commercial reagents cannot transfect them and cationic polymers show only moderate success. The specific intracellular barriers limiting non-viral gene delivery to human T cells are poorly understood, hindering rational polymer design…
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