Papers, explained in our own words

1. Protein and Antibody Delivery into Difficult-to-Transfect Cells by Polymeric Peptide Mimics

Coralie M. Backlund, Christopher R. Hango, Lisa M. Minter, Gregory N. Tew

Intracellular protein and antibody delivery is limited by inefficient carriers, high toxicity, and a frequent requirement for serum-free conditions, especially in difficult-to-transfect cells such as immune cells, stem cells, and neurons. A simple noncovalent carrier that works in complete media and across diverse cell types is needed. Commercial carriers: Xfect delivered GFP to all five cell types only in serum-free media; neurons were most challenging. PULSin delivered to all except neurons in serum-free media. ProteoJuice achieved only ~20%.