Methods in molecular biology (Clifton, N.J.)2027ResearchNon-viral Gene Delivery
1. Improvement of Genome Editing Efficiency in Mammalian Cells by a Refined Uni-Vector Prime Editing System
Liao Lz, Wu Kj
Prime editing is an advanced technology in the CRISPR/Cas genome editing field. The original multi-vector method utilized in prime editing has less efficiency and precision and is also inherently difficult in generating homozygous mutations in mammalian cells. To overcome these technical difficulties, a Uni-vector…
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