Papers, explained in our own words

1. Integration of Charged Peptide Amphiphiles within Lipid Nanoparticles (LNPs) for Enhanced Targeted mRNA Delivery

Yuan Y, Li G, Liu Y, Liu L, Hu C, Qi H, Et Al.

Lipid nanoparticles (LNPs) play a pivotal role in mRNA-based therapy; however, achieving targeted delivery remains challenging. We developed an LNP platform integrating peptide amphiphiles (PAs) to exploit their dual bioactivity and electrostatic tunability. Two PAs, cationic C16TAT (derived from a cell-penetrating…

• cell-penetrating peptides
• co-assembly
• lipid nanoparticles
• peptide amphiphiles
• targeted delivery

2. Advances in Peptide-Functionalized Polymeric Nanocarriers for Cancer Therapy

Ommi O, Covarrubias-Zambrano O, Bossmann Sh, Kamath D

Background/Objectives: Peptide-functionalized polymeric nanocarriers are redefining targeted cancer therapy by combining the molecular specificity of peptides with the structural versatility of polymers. This review aims to highlight recent advances in peptide-functionalized polymeric nanocarrier systems designed to…

• cancer
• cell-penetrating peptides
• nanocarriers
• polymer
• targeting peptides
• therapeutic peptides
• tumor-microenvironment

3. Liposomal Delivery Systems: Design Optimization and Current Applications

Liposomes are attractive pharmaceutical carriers because they encapsulate hydrophilic and hydrophobic drugs and are biocompatible and biodegradable. However, conventional liposomes suffer from aggregation/fusion, premature payload release, rapid clearance by the mononuclear phagocyte system (MPS), low entrapment…

• Liposomes
• siRNA
• Peptides
• Nucleic acids
• Lipid nanoparticle
• Endosomal escape
• Cell-penetrating peptides

4. Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Translocation of Complexed siRNA

Skye Zeller, Chang Seon Choi, Pradeep D. Uchil, Hong-Seok Ban, Alyssa Siefert, Tarek M. Fahmy, Walther Mothes, Sang-Kyung Lee, Priti Kumar

Arginine-rich cell-penetrating peptides (CPPs) such as nona-arginine (9R) can effectively translocate covalently attached biomolecules into cells, but poorly deliver electrostatically complexed siRNA, with most CPP:siRNA complexes trapped in endosomes. The mechanism by which ligand attachment to 9R overcomes this…

• siRNA
• Peptides
• Cell-penetrating peptides
• T cells
• Transfection
• mRNA
• Polymeric

5. Modeling the endosomal escape of cell-penetrating peptides using a transmembrane pH gradient

Fatemeh Madani, Rania Abdo, Staffan Lindberg, Hisaaki Hirose, Shiroh Futaki, Ulo Langel, Astrid Gräslund

Cell-penetrating peptides (CPPs) can internalise cells with biologically active cargo, but endosomal entrapment is a major limitation for cytoplasmic delivery, as cargo molecules must escape the endosome before degradation and acidification. While CPPs are known to enter cells via endocytosis, the background…

• Endosomal escape
• Peptides
• Cell-penetrating peptides
• Endocytosis
• Nanocarriers
• Gene delivery
• Drug delivery

6. Uptake of cell-penetrating peptides is dependent on peptide-to-cell ratio rather than on peptide concentration

Mattias Hallbrink, Johannes Oehlke, Gisela Papsdorf, Michael Bienert

Cell-penetrating peptides (CPPs) efficiently translocate across plasma membranes and are promising delivery vectors for therapeutic macromolecules. However, the influence of cell state, density, and peptide-to-cell ratio on CPP uptake and degradation has received little attention. Understanding whether CPP uptake is…

• Peptides
• Cell-penetrating peptides
• Endocytosis
• Nucleic acids
• Nanocarriers
• Gene delivery
• Drug delivery