Papers, explained in our own words

145. Optical Manipulation-Assisted Three-Dimensional Single-Particle Tracking Reveals Extracellular Vesicle-Cell Real-Time Interactions In Vitro

Wang C, Huang M, Zhang Y, Chen X, Li S, Chen Q, Et Al.

Extracellular vesicles (EVs) play essential roles in intercellular communication and are promising nanocarriers for drug delivery. Directly resolving the three-dimensional (3D) dynamics of EVs during their interactions with target cells can provide critical insights and help guide the rational design of EV-based…

• double-helix point spread function
• dynamic uptake
• extracellular vesicles
• optical tweezers
• single-particle tracking

146. Nanoparticle delivery systems for gene therapy of degenerative musculoskeletal diseases

Chen X, Sun J, Xie W, Arthur Vithran Dt, Zhang Q, Rosenholm Jm, Et Al.

Degenerative musculoskeletal diseases (DMDs) are a group of chronic conditions characterized by the progressive deterioration of tissue structure and function, primarily affecting the articular cartilage, bones, tendons, and ligaments. With global aging populations, the rising prevalence of these disorders presents a…

• Ageing
• Degenerative musculoskeletal diseases
• Delivery systems
• Gene therapy
• Nanoparticles
• Osteoarthritis

147. Targeted delivery of celastrol and siRNA via macrophage biomimetic nanoparticles for doxorubicin-induced cardiomyopathy therapy

Zhang Q, Guo T, Xue X, Zhang Z, Liu X, Cai Q, Et Al.

Doxorubicin-induced cardiomyopathy (DIC) is a severe complication following anthracycline chemotherapy, and its main pathogenesis is inflammatory responses and ferroptosis. This study constructed hyaluronic acid-based macrophage biomimetic nanoparticles (M@HCDA) to co-delivery of Celastrol (Cel) and siADAM17 for the…

• ADAM17
• Biomimetic nanoparticle
• Celastrol
• Doxorubicin-induced cardiomyopathy
• Inflammation/ferroptosis

148. A dual-targeting lipid nanoparticle delivery system for precision phage therapy against multidrug-resistant Gram-negative bacteria

Chen Z, Hu W, Nie H, Zhang Y, Tang W, Yu Z, Et Al.

The escalating crisis of antimicrobial resistance, particularly among Gram-negative bacteria, demands innovative therapeutic strategies. Here, we present a dual-targeting lipid nanoparticle-chimeric phage (AbLCP) system engineered to combat multidrug-resistant (MDR) Gram-negative bacteria. Our design integrates a…

149. Laser-responsive chitosan-coated solid lipid nanoparticles co-encapsulating CaO 2 nanoparticles and IR780 for on-demand and enhanced photodynamic therapy of cancers

Amiri M, Heydari E, Esmaeili M, Zare-Behtash H, Moeini M

The photodynamic therapy (PDT) efficacy is limited by tumor hypoxia and inefficient intracellular accumulation of photosensitizers (PSs). This study reports chitosan-coated solid lipid nanoparticles (SLNs) co-encapsulating the near-infrared photosensitizer IR780 and oxygen-generating CaO 2 nanoparticles (NPs) for…

• Chitosan
• Light-responsive
• Oxygen release
• Photodynamic therapy
• Solid lipid nanoparticles

150. Redox-Induced Polymeric Micelle-to-Coacervate Transition for Enhanced Endosomal Escape, Prolonged Drug Retention, and Improved Sequential Drug Delivery

Ying J, Wang J, Liu Z, Chen Y, Nie Y, Huang Y, Et Al.

Polymeric micelles (PMs) and coacervate microdroplets (CMs) are both promising delivery vehicles, yet each suffers from distinct limitations. The delivery efficiency of PMs is often hindered by factors, such as limited endosome escape and uncontrolled drug release. On the other hand, CMs are prone to coalescence and…

151. Near-Infrared Light-Responsive Liposomes for Modulating Switchable CAR-T Cell Therapy

Lu G, Zhang H, Wang H, Gao X, Zhang K, Song Y, Et Al.

Chimeric antigen receptor T (CAR-T) cell therapy has great potential but is limited by off-target effects and cytokine release syndrome. To address these challenges, we developed a mesothelin-targeted switchable CAR-T cell system whose activity can be externally controlled by near-infrared (NIR) light-responsive…

• chimeric antigen receptor T
• liposomes
• mesothelin
• near-infrared light-responsive

152. Smart nanocarriers and biomaterials for targeted drug delivery in spinal cord injury: molecular mechanisms and translational perspectives

Han W, Qi P, Li J, Min L, Li H, Yin H

Spinal cord injury (SCI) results in permanent motor, sensory, and autonomic dysfunction below the level of injury; currently, there are no effective treatments to promote neural functional recovery. Inflammatory responses, oxidative stress, apoptosis, excitotoxicity, glial scarring, and impaired axonal…

• drug delivery systems
• mechanisms of action
• nanocarriers
• nerve regeneration
• spinal cord injury
• targeted therapy

153. LNP-mediated CRISPR gene editing of human vascular endothelial cells in 2D and 3D microfluidic cell culture systems

Olsen Al, Brandt Cbv, Larsen Rk, Fonager Sv, Zubia-Aranburu J, Haskó J, Et Al.

Efficient and cell-type-specific delivery remains a major barrier to CRISPR-based genome editing in vascular endothelial cells. Here, we systematically evaluate lipid nanoparticle (LNP)-mediated mRNA and CRISPR delivery in human umbilical vein endothelial cells (HUVECs). While a conventional SM-102-based LNP…

• CRISPR
• Cas9
• LNP
• MT: delivery strategies
• endothelial cells
• endothelium
• genome editing
• lipid nanoparticles
• microfluidics
• microvasculature on a chip

154. Overcoming technological and biological barriers to pulmonary mRNA delivery with lipid-polymer hybrid nanoparticles

Biesel A, Shandheep A, Pavlou A, Busker Lm, Koch M, Kopliku V, Et Al.

Delivering mRNA to the lungs promises treatment of genetic, infectious and non-communicable lung diseases. Yet, conventional lipid nanoparticle formulations lack the stability needed to endure the harsh mechanical stress during nebulization. Additionally, once deposited in the respiratory tract, gene delivery systems…

• DLin-MC3-DMA
• DOTAP
• Lung epithelial cells
• Microfluidics
• Poly(lactic-co-glycolic) acid
• Vibrating mesh nebulization

155. Liposomal and liposome-based formulations of pentacyclic triterpenoids in cancer nanomedicine: A critical appraisal of preclinical progress and future perspectives

Babaei M, Miroliaei M

Pentacyclic triterpenoids (PTs) comprise a broad range of multifunctional plant-derived compound that serve as attractive scaffolds for the development of anticancer drugs. Their modes of action range from triggering apoptosis to modulating oncogenic signaling pathways and the tumor microenvironment. Despite these…

• Betulinic acid
• Celastrol
• Glycyrrhizic acid
• Lipid nanocarriers
• Targeted drug delivery
• Ursolic acid

156. Review Article: mRNA CARs: An Emerging New-Old Idea

Wolf Y

Chimeric antigen receptor (CAR) T cell therapy has transformed cancer immunotherapy. Still, conventional viral vectors carry risks of insertional mutagenesis, are costly to manufacture, and can cause persistent on-target/off-tumor toxicity. Messenger RNA (mRNA) platforms offer a nonintegrating, transient alternative…

157. Thermodynamic Model and Experiments Indicate Kinetically Trapped States in Ionisable Lipid Nanoparticles

Kostyurina E, Sudarsan A, Huber M, Westerhausen C, Schwierz N, Raedler J, Et Al.

Lipid nanoparticles (LNPs) are the leading delivery vehicles for RNA-based therapies, with pH-dependent mesophase transitions of their ionizable lipids proposed to play a crucial role in LNP function and endosomal escape. However, a consistent quantitative framework linking molecular lipid properties to the…

158. T cell engineering by targeted small extracellular vesicles delivering CRISPR/Cas9 system for PD-1 knock-out

Farahani Ms, Shamsara M, Moazzeni Sm, Khalili S, Johnson B, Darzi L, Et Al.

Among the emerging cancer therapies, immunotherapy using T cell engineering stands out as one of the most promising approaches. While the clustered regularly interspaced palindromic repeats (CRISPR/Cas) system is widely recognized as one of the most common gene-editing system tools, the main challenge for in vivo…

• PD-1 knock-out
• CRISPR/Cas9
• Gene editing
• T cell
• Targeted small extracellular vesicle

159. Exosome-Coated Dendrimer Nanogels as a Therapeutic Vaccine for CT Imaging-Guided Chemoimmunotherapy of Pancreatic Cancer

Li Z, Xiao X, Li Y, Qin M, Xia L, Yang J, Et Al.

Development of new medicines that can treat pancreatic cancer with a sustained immune memory effect remains to be a challenging task. Herein, we report a tumor microenvironment-responsive dendrimer nanogel (DNG)-based therapeutic vaccine co-loaded with toyocamycin (Toy) and gold nanoparticles (Au NPs) and the surface…

• chemoimmunotherapy
• dendrimer nanogels
• exosomes
• immunogenic cell death
• pancreatic cancer

160. Formulation optimisation of an mRNA-lipid nanoparticle platform sustains in vivo production of the SARS-CoV-2-neutralizing nanobody VHH 72

Dong Y, Li Q, Ou Y, Li M, Liu N, Wang X, Et Al.

Neutralizing antibodies act rapidly against SARS-CoV-2, but monoclonal antibodies are costly to manufacture and single-domain antibodies are cleared rapidly. Here we evaluated an mRNA-lipid nanoparticle (LNP) platform encoding the neutralizing nanobody VHH 72 and optimized the carrier by raising DSPC from 10 to 40…

• Formulation optimization
• Lipid nanoparticle
• Nanobody
• SARS-CoV-2
• Sustained expression
• mRNA delivery

161. Comparison of Wildtype and Recombinant Adeno-Associated Virus (AAV) Production Reveals Distinct Productivity and Content Ratio Profiles

Alfieri S, Lee Kh

Recombinant adeno-associated virus is a leading viral vector for gene therapy. However, recombinant adeno-associated virus production systems have low titer and content ratio (ratio of genome-containing to total adeno-associated virus capsids), making it difficult to generate the large number of vector genomes…

• content ratio
• gene therapy
• recombinant adeno‐associated virus (rAAV)
• transient transfection
• viral vector manufacturing
• wildtype AAV infection

162. Simple heat-assisted method for mRNA loading into empty lipid nanoparticles enables robust in vitro and in vivo delivery

Miyazaki T, Kubara K, Kondo K, Kamiyama H, Hara S, Moriya J, Et Al.

mRNA-lipid nanoparticles (LNP) have emerged as a versatile modality, enabling diverse applications by changing encoded mRNA sequences. However, the conventional mRNA-LNP preparation process poses significant barriers to non-specialists because it requires specialized equipment and technical expertise. In this study…

• 96-well compatible
• Homogeneity
• Lipid nanoparticle
• Post-encapsulation
• Technical barriers
• mRNA

163. Maleimide availability in thiol-maleimide peptide conjugation on PEGylated liposomes: a reactive-site accounting framework illustrated using hepatic drug-delivery systems

Maroof K, Genç R

Peptide-targeted PEGylated liposomes are a widely investigated active-targeting strategy for hepatocellular carcinoma (HCC) and other hepatic indications. A critical but underreported formulation variable is effective maleimide availability: the number of outward-facing, unhydrolysed, sterically accessible maleimide…

• DSPE-PEG-maleimide
• conjugation efficiency
• hepatic nanomedicine
• hepatocellular carcinoma
• maleimide availability
• peptide-targeted liposomes
• thiol-maleimide conjugation

164. Natural killer cell-derived extracellular vesicles in cancer: mediators of intercellular communication and tumor immune signaling

Liu J, Yang Y, Zhang W, Liu J, Yu Y, Song Y, Et Al.

Natural killer (NK) cells are essential components of innate immunity and play a pivotal role in tumor surveillance through direct cytotoxicity and immune regulation. In recent years, NK-cell-derived extracellular vesicles (NK-EVs) have emerged as a promising cell-free therapeutic modality that preserves key…

• intercellular communication
• miRNA-mediated regulation
• natural killer cell-derived extracellular vesicles (NK-EVs)
• tumor immune signaling
• tumor microenvironment

165. Neoantigen nanovaccines for cancer immunotherapy: design strategies, translational bottlenecks, and clinical prospects

Sun M, Xiao L, Yu Y

Neoantigen vaccines have become an important direction in cancer immunotherapy because mutation-derived tumor antigens can induce highly specific T-cell responses while minimizing immune tolerance associated with tumor-associated antigens. However, their therapeutic performance remains limited by several…

• Antigen delivery
• Cancer immunotherapy
• Clinical translation
• Nanovaccine
• Neoantigen

166. Engineered Magnetic Nanoparticles for Dual-Modality Cancer Therapy: Advances in Hyperthermia and Magnetically Controlled Drug Delivery

Murugan S, Ashokkumar M

Cancers remain among the most lethal diseases worldwide, accounting for a substantial proportion of human mortality. Despite significant advances, conventional therapeutic strategies are often limited by tumor microenvironment-mediated resistance, non-specific biodistribution, and severe systemic toxicity…

• cancer treatment
• magnetic hyperthermia
• magnetically triggered drug delivery
• self-regulated heating

167. Apolipoproteins in nanoparticle-mediated brain drug delivery: promise, mechanisms, and misconceptions

Gelperina S, Wacker Mg

Apolipoprotein E (apoE) plays a context-dependent role in nanomedicine for central nervous system drug delivery. It can facilitate cerebral endothelial recognition and increase brain exposure in nanoparticle systems while simultaneously promoting hepatic uptake, challenging its traditional interpretation as a…

• Apolipoprotein E
• blood-brain barrier
• brain drug delivery
• lipid nanoparticles
• liver tropism
• low-density lipoprotein receptor family
• protein corona
• receptor-mediated transcytosis

168. Bioactivatable conjugated nanocarriers for synergistic mRNA-mediated cancer immunotherapy

Nazir N, Gajjar Tb, Hanumanthayya M, Bainsal N, Singhal D, Smerat A, Et Al.

mRNA immunotherapy holds transformative potential in oncology, yet single-agent approaches frequently fail against immunosuppressive tumor microenvironments (TMEs). Bioactivatable co-delivery of immunogenic chemotherapy alongside mRNA offers mechanistically synergistic advantages, yet coformulation of hydrophobic…

• Immunogenic cell death
• Lipid nanoparticles
• ROS-responsive prodrug
• Tumor microenvironment
• cGAS-STING
• mRNA cancer vaccine