Accounts of chemical research2026ResearchNon-viral Gene DeliveryDrug Delivery
Qu F, Thompson Dh
Gene therapy has evolved into a cornerstone modality in modern medicine, with applications spanning antisense oligonucleotide treatments, RNA interference therapies, mRNA vaccines, in vivo CAR-T cell therapies, and CRISPR-mediated in vivo gene editing. The demands of this proliferating therapeutic approach compel the…
Read the article →Drug development and industrial pharmacy2026ReviewNon-viral Gene DeliveryDrug Delivery
Joseph At, Ravindran S
This review critically examines phospholipids as pharmaceutical excipients, focusing on physicochemical properties, self-assembly behavior, drug delivery applications, translational limitations, and future opportunities in customised therapeutics. Phospholipids have evolved from inert excipients into multifunctional…
Read the article →Science advances2026ResearchNon-viral Gene DeliveryDrug Delivery
Xiao H, Li X, Cai K, Leng Y, Miao X, Yin T, Et Al.
Liver fibrosis is a severe complication of diverse chronic liver diseases characterized by a dysregulated hepatic microenvironment, and effective clinical therapeutic strategies for this condition remain lacking to date. Chimeric antigen receptor macrophages (CAR-Ms) hold considerable therapeutic potential for liver…
Read the article →International journal of pharmaceutics2026ReviewNon-viral Gene DeliveryDrug Delivery
Shen Y, Ye X, Shen S, Lv F
Chimeric antigen receptor (CAR)-engineered natural killer (NK) cell therapy has emerged as a promising immunotherapeutic strategy for cancer treatment, offering advantages over CAR-T cells such as a lower risk of cytokine release syndrome and graft-versus-host disease and the potential for off-the-shelf manufacturing.
Read the article →Theranostics2026ReviewNon-viral Gene DeliveryDrug Delivery
Wu X, Kou X, Bai L, Liu C, Wu Q, Zhang M, Et Al.
Clustered regularly interspaced short palindromic repeats (CRISPR), a precise genome-editing platform, has revolutionized genetic engineering. The highly selective and programmable characteristics of CRISPR have enabled its broad application. Recent advances in CRISPR biotechnology have promoted its widespread use in…
Read the article →International journal of pharmaceutics2026ReviewNon-viral Gene DeliveryDrug Delivery
Shin Y, Lee S, Nguyễn Ánh Nt, Chung M
Lipid nanoparticles (LNPs) represent clinically validated non-viral platforms for nucleic acid delivery, yet expanding their application toward precision gene therapy demands more than efficient particle formulation or tissue accumulation. Functional delivery requires navigating a sequential biological cascade…
Read the article →Frontiers in oncology2026ReviewNon-viral Gene DeliveryDrug Delivery
Wu S, Liao H, Zhang W
Therapeutic mRNA vaccines are a clinically validated cancer immunotherapy platform using in vitro transcribed mRNA encoding tumor antigens to induce durable anti-tumor immunity. Advances in mRNA engineering, lipid nanoparticle (LNP) delivery, and immunomodulatory combinations have propelled this approach from…
Read the article →Nano letters2026ResearchNon-viral Gene DeliveryDrug Delivery
Liu H, Yang B, Chen S, Li Y, Zhang Y, Huang Y, Et Al.
Highly efficient lung-selective mRNA delivery is pivotal for advancing mRNA-based targeted therapy. Here, we report bis(phosphoramidate diester)-based ionizable cationic lipids (bpLs) for lung-specific mRNA delivery via a distinct intercellular transport mechanism. A bpL library is synthesized by modular headgroups…
Read the article →Frontiers in immunology2026ResearchNon-viral Gene DeliveryDrug Delivery
Li J, Goncalves B, Melamed G, O'neill D, Cozzone R, Iuliucci S, Et Al.
Lentivirus (LV)-based manipulation of immune cells is a commonly used approach in cell therapies. However, the most frequently used Vesicular Stomatitis Virus Glycoprotein (VSV-G) pseudotyping approach is inefficient to support LV gene delivery of mouse immune cells due to the lack of an optimal entry receptor. Here…
Read the article →Discover genetics and evolution2026ReviewNon-viral Gene Delivery
Bhardwaj A
Off-target activity remains a significant challenge in the clinical development of CRISPR-based therapies. As programmable nucleases advance from experimental tools toward approved medicines, the ability to predict, detect, and mitigate unintended genomic editing events has acquired direct translational importance.
Read the article →Acta pharmaceutica (Zagreb, Croatia)2026ResearchNon-viral Gene DeliveryDrug Delivery
Li N, Qu Z, Du D
The application of nanotechnology in drug delivery systems has attracted considerable scientific interest, particularly with respect to improving the therapeutic efficacy and safety profiles of pharmacological agents. This review critically examines nanotechnology-based drug delivery systems for the management of…
Read the article →Expert opinion on drug delivery2026ReviewNon-viral Gene DeliveryDrug Delivery
Wang N, Meng L, Shi Jx, Bartneck M
Small interfering RNA (siRNA) has progressed from an experimental gene-silencing tool to an established therapeutic modality through advances in chemical modification and delivery. Lipid nanoparticles (LNP) and N-acetylgalactosamine (GalNAc)-siRNA conjugates have driven this clinical translation, particularly for…
Read the article →ACS nano2026ResearchNon-viral Gene DeliveryDrug Delivery
He Y, Wang L, Williams Ap, Valerio Ti, Sun Y, Bowman J, Et Al.
In this study, we developed a library of mRNA lipid nanoparticles (LNPs) containing clinically used ionizable lipids. We surface-modified these nanoparticles with heparosan polysaccharides and systematically compared their performance with conventional poly(ethylene glycol) (PEG)-modified formulations. Our heparosan…
Read the article →ACS nano2026ResearchNon-viral Gene DeliveryDrug Delivery
Abbasi S, Bockley Km, Dohr J, Chen E, Carter Dm, Berlinicke Ca, Et Al.
Active targeting can improve the efficacy of mRNA therapeutics, but ligand-mediated targeting with lipid nanoparticles (LNPs) remains challenging. Here, we studied the impact of polyethylene glycol (PEG)-lipid composition and triantennary N-acetylgalactosamine (TriGalNAc)-modification on the hepatic delivery…
Read the article →bioRxiv : the preprint server for biology2026ResearchNon-viral Gene DeliveryDrug Delivery
Ansong-Ansongton Y, Adanho Csa, Goreke U, Lawanprasert A, Vysotskiy M, Tang Y, Et Al.
Hemoglobinopathies, including sickle cell disease (SCD) and thalassemia syndromes, affect millions of individuals worldwide who have limited access to curative therapies. Autologous hematopoietic stem cell transplant following ex vivo CRISPR editing of the BCL11A erythroid enhancer reactivates fetal hemoglobin (HbF)…
Read the article →Molecular neurobiology2026ReviewNon-viral Gene DeliveryDrug Delivery
Liu S, Lu J, Liu J, Liu H, Liu Z, Yang D, Et Al.
The scarcity of effective therapies for Alzheimer's disease (AD) underscores the urgent need for innovative strategies. This review focuses on the targeted delivery of engineered small extracellular vesicles (sEVs, 30-150 nm). By capitalizing on their intrinsic properties as natural nanocarriers-including low…
Read the article →International journal of nanomedicine2026ReviewNon-viral Gene DeliveryDrug Delivery
Thalij Km, You Hw, Habeeb M, Ratchagar Jv, Khot Vs, Gorde Pl
Hepatocellular carcinoma is the sixth most common cancer worldwide and a leading cause of cancer related mortality. Risk factors include chronic hepatitis B/C, obesity, alcohol abuse, diabetes and metabolic disorders. In advanced stages, normal treatments such as surgery, transplantation and chemotherapy are not as…
Read the article →Molecular therapy. Nucleic acids2026ResearchNon-viral Gene DeliveryDrug Delivery
Qi R, Chen H, Chen R, Bao H, Sun Y, Xu R, Et Al.
Targeted delivery and controlled expression of mRNA encapsulated by lipid nanoparticles (mRNA-LNPs) are critical for the development of safe and effective mRNA medicines. However, efficient post-delivery regulation of mRNA-LNP expression remains challenging, and conventional approaches largely rely on modifying the…
Read the article →Bioengineering & translational medicine2026ResearchNon-viral Gene DeliveryDrug Delivery
Wang S, Jing S, Zou S, Zhang Y, Ge M, Gao K, Et Al.
HER2 overexpression is a pivotal oncogenic alteration in NSCLC and is associated with poor prognosis. Existing HER2-targeted therapeutics, including antibody-drug conjugates, have limited anticancer efficacy and prominent systemic toxicity, whereas radiotherapy-mediated tumor immunosuppression further hinders…
Read the article →ACS applied bio materials2026ResearchNon-viral Gene DeliveryDrug Delivery
Wang W, Liu H, Tang W, Shin J, Kim Yc, Li S
Lipid nanoparticles (LNPs) are widely explored as delivery vehicles for self-amplifying RNA (saRNA); however, incorporation of surface functionalities, such as fluorescent dyes for imaging and ligands for targeted delivery, remains challenging. In this study, we introduce a reactive poly(pentafluorophenyl acrylate)…
Read the article →Journal for immunotherapy of cancer2026ResearchNon-viral Gene DeliveryDrug Delivery
Rehman Au, Khan Mn, Akhtar A, Qin Q, Zhang X, Asghar Ma, Et Al.
Interleukin-7 receptor (IL7R) signaling is critical for T-cell survival, homeostasis, and immune function, but the therapeutic potential of increasing IL7R expression through messenger RNA (mRNA) delivery remains poorly defined. We investigated whether IL7R mRNA therapy could enhance antitumor immunity and improve…
Read the article →Biomaterials science2026ResearchNon-viral Gene Delivery
Neri-Cruz Ce, Carta D, Gautrot Je
Rational design of polycationic gene delivery vectors requires understanding how polymer chemistry governs RNA complexation, cellular uptake, and intracellular release kinetics. Poly(dimethylaminoethyl methacrylate) brushes were found to display striking potential for the delivery of siRNA, with high knockdown…
Read the article →Drug metabolism and bioanalysis2026ReviewNon-viral Gene DeliveryDrug Delivery
Ansari I, Yadav S, Prajapati S
In regenerative medicine, biomaterial-based medication delivery systems are a gamechanger because they allow for the restoration of function and the healing of damaged tissues. This study aims to examine the potential of synthetic and natural biomaterials for the delivery of medications and the regeneration of…
Read the article →Nanotechnology2026ResearchDrug Delivery
Le Th, Phan Lmt
Extracellular vesicles (EVs), particularly stem cell-derived exosomes, serve as vital natural nanocarriers in regenerative medicine, immune modulation, and targeted drug delivery due to their innate ability to transport bioactive cargo across complex biological barriers. Despite this potential, the therapeutic…
Read the article →