Molecular cancer2026ResearchNon-viral Gene DeliveryDrug Delivery
Ni J, He G, Wang H, Qin Y, Guo J, Wang Y, Et Al.
Proficient mismatch repair/microsatellite-stable colorectal cancer (pMMR/MSS CRC), which represents the majority of clinical cases, exhibits low tumor mutational burden and minimal responsiveness to immune checkpoint blockade. Disrupting the MMR system has been shown to resensitize pMMR/MSS CRC to immune…
Read the article →Journal of controlled release : official journal of the Controlled Release Society2026ResearchNon-viral Gene DeliveryDrug Delivery
Kim M, Jeong M, Chatterjee S, Vaidya A, Pham C, Lee Sm, Et Al.
Fibroblast activation and differentiation into myofibroblasts drive excessive extracellular matrix deposition and irreversible scarring in pulmonary fibrosis, such as idiopathic pulmonary fibrosis (IPF), underscoring the need for therapies that directly target fibroblast activation. Here, we apply engineered…
Read the article →Advanced science (Weinheim, Baden-Wurttemberg, Germany)2026ResearchNon-viral Gene DeliveryDrug Delivery
Sarode A, Patel P, Derosa F, Karve S, Gervier R
The clinical translation of mRNA-lipid nanoparticle (mRNA-LNP) therapeutics is constrained by limited stability of liquid formulations, necessitating ultra-cold storage and restricting global accessibility. Here, we report a spray-dried dry powder platform for mRNA-LNPs that overcomes these limitations while enabling…
Read the article →European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V2026ResearchDrug Delivery
Da Costa Rodrigues T, Kola Ns, Patel D, Ibrahim A, Ma'aruf Sy, Haddadi A, Et Al.
Subunit vaccines are typically given through injections, which limits mucosal immunity. Most adjuvants do not stimulate mucosal responses but using lipid nanoparticles (LNPs) encapsulating polyinosinic-polycytidylic acid (poly(I:C)), a toll-like receptor 3 agonist, could enhance the immune response in the lungs. We…
Read the article →Biomacromolecules2026ResearchDrug Delivery
Hall Cb, Tan L, Okai Dna, Dobler S, Adigun R, Okuwobi Cb, Et Al.
Herein, we characterize a class of PAMAM/acyl synthetic lipids and their self-assembled nanostructures. We explicitly determined the impact of hydrocarbon length, saturation, and synthetic lipid volume on the properties of the self-assembled, single-component nanostructure. The synthesis of the PAMAM-acyl lipid…
Read the article →International journal of pharmaceutics: X2026ResearchNon-viral Gene DeliveryDrug Delivery
Kim Kh, Mainali A, Pih Sh, Jung Hs, Kim Hj, Kim Na, Et Al.
RNA-loaded lipid nanoparticles (LNPs) are highly sensitive to temperature fluctuations, which present significant challenges for storage and transportation. Lyophilization is a potential strategy to enhance storage stability; however, the freeze-drying process can subject nanoparticles to stresses that may alter…
Read the article →ACS applied materials & interfaces2026ResearchNon-viral Gene DeliveryDrug Delivery
Quagliarini E, Petronella F, Digiacomo L, Miglietta S, Buccini L, Mura F, Et Al.
Gene therapy is a cutting-edge strategy for treating, preventing, and potentially eradicating a wide range of diseases. Its success is critically dependent on the transfection efficiency of gene delivery systems. In this context, lipid nanoparticles (LNPs) are widely investigated for the delivery of plasmid DNA…
Read the article →Journal of controlled release : official journal of the Controlled Release Society2026ResearchNon-viral Gene DeliveryDrug Delivery
Kehrein J, Reus E, Holick Ct, Mummel S, Ates I, Wang X, Et Al.
Delivering therapeutic mRNA relies on lipid nanoparticles (LNPs). Finding optimal process parameters for new lipid combinations in LNP formulations remains a challenge. We used an automated, self-regulating microfluidic formulation platform developed for PEG-containing LNPs that actively changes process parameters to…
Read the article →Journal of ovarian research2026ResearchNon-viral Gene DeliveryDrug Delivery
Xu Jy, Liu K, Chen Ly, Liu C, Chen Dy, Wu Sg, Et Al.
Ovarian cancer is the foremost cause of mortality among gynecologic malignancies. Paclitaxel (PTX) exerts a significant therapeutic effect on ovarian cancer by targeting microtubules and impeding the cell cycle progression during the mitosis phase. MOB kinase activator 1 A (MOB1A) has been proven to regulate the…
Read the article →Molecular pharmaceutics2026ResearchNon-viral Gene DeliveryDrug Delivery
Gul S, Vergnaud J, Wang Q, Hery M, Dumont F, Tran P, Et Al.
Tumor-associated macrophages (TAMs) are central regulators of the tumor microenvironment (TME). Their polarization state can critically influence tumor development and treatment resistance. In the TME, the pro-tumor M2-like phenotype is the most prevalent among TAMs and contributes to tumor progression by promoting…
Read the article →ACS applied materials & interfaces2026ResearchNon-viral Gene DeliveryDrug Delivery
Yuan Y, Li G, Liu Y, Liu L, Hu C, Qi H, Et Al.
Lipid nanoparticles (LNPs) play a pivotal role in mRNA-based therapy; however, achieving targeted delivery remains challenging. We developed an LNP platform integrating peptide amphiphiles (PAs) to exploit their dual bioactivity and electrostatic tunability. Two PAs, cationic C16TAT (derived from a cell-penetrating…
Read the article →Molecular therapy. Nucleic acids2026ResearchNon-viral Gene DeliveryDrug Delivery
Olsen Al, Brandt Cbv, Larsen Rk, Fonager Sv, Zubia-Aranburu J, Haskó J, Et Al.
Efficient and cell-type-specific delivery remains a major barrier to CRISPR-based genome editing in vascular endothelial cells. Here, we systematically evaluate lipid nanoparticle (LNP)-mediated mRNA and CRISPR delivery in human umbilical vein endothelial cells (HUVECs). While a conventional SM-102-based LNP…
Read the article →The Journal of pharmacology and experimental therapeutics2026ResearchNon-viral Gene DeliveryDrug Delivery
Nazir N, Gajjar Tb, Hanumanthayya M, Bainsal N, Singhal D, Smerat A, Et Al.
mRNA immunotherapy holds transformative potential in oncology, yet single-agent approaches frequently fail against immunosuppressive tumor microenvironments (TMEs). Bioactivatable co-delivery of immunogenic chemotherapy alongside mRNA offers mechanistically synergistic advantages, yet coformulation of hydrophobic…
Read the article →ACS applied bio materials2026ResearchDrug Delivery
Hou S, Zhou D, Zhang Y, Li Y, Fan D, Hu Y, Et Al.
Therapeutic peptide vaccines against HPV-associated cancers are often limited by insufficient immunogenicity and inefficient intracellular delivery. Here, we develop a phenolic hydroxyl-containing lipid nanoparticle (EMT@LNP) as a nanomedicine platform for the coordinated codelivery of HPV16 E6/E7 peptide antigens…
Read the article →Advanced healthcare materials2026ResearchNon-viral Gene DeliveryDrug Delivery
Ren Y, Wang Q, Li P, Zhang Z, Wei G, Tan Y, Et Al.
Lipid nanoparticle (LNP)-enabled genetic medicines are reshaping cancer therapy yet remain constrained by liver-biased tropism, dose-limiting toxicities, and limited efficacy in refractory solid tumors such as pancreatic ductal adenocarcinoma (PDAC). Here, exosome-inspired proteolipid nanoparticles (Em-pLNPs) are…
Read the article →Angewandte Chemie (International ed. in English)2026ResearchNon-viral Gene DeliveryDrug Delivery
Streiber M, Holick Ct, Gath L, Tsarenko E, Bachmann V, Litsiou S, Et Al.
Efficient, biocompatible, and flexible application of CRISPR-Cas9 remains a major challenge in gene therapy. This study addresses a novel formulation method, focusing on organic solvent-free lipid nanoparticles for the efficient delivery of CRISPR-Cas9 components. Therefore, novel stealth lipids comprising the lipid…
Read the article →Biomedicines2026ResearchNon-viral Gene DeliveryDrug Delivery
Zahedipour F, Sadeghianfard S, Wisser S, Dinarvand M, Steiner D, Ramesh A, Et Al.
Background/Objectives: Glioblastoma multiforme (GBM) remains the most aggressive and treatment-refractory primary brain tumor, with current therapeutic modalities providing minimal survival improvement. Chimeric antigen receptor (CAR)-based immunotherapies succeeded in hematologic malignancies but are constrained in…
Read the article →International journal of pharmaceutics2026ResearchNon-viral Gene DeliveryDrug Delivery
Li J, Li C, Wang H, Yu H, Zhang T, Li C, Et Al.
Osteoarthritis (OA) progression involves cartilage matrix loss and persistent inflammation. This study developed a machine learning-guided and chondrocyte membrane-camouflaged lipid nanoparticle carrying messenger RNA (CM-LNP@mRNA) platform for intra-articular SOX9 delivery. Random forest analysis identified the…
Read the article →Nano letters2026ResearchNon-viral Gene DeliveryDrug Delivery
Zhu P, Huang Y, Pan L, Leng Y, Zheng H, Zhou Y, Et Al.
Atherosclerotic vulnerable plaques pose a risk for acute coronary syndrome and sudden cardiac death due to plaque rupture and thrombosis. While nanomedicines modulating macrophage functions have attracted intensive interest, efficient delivery to plaque macrophages remains challenging. Here, mannose-modified lipid…
Read the article →Journal of controlled release : official journal of the Controlled Release Society2026ResearchNon-viral Gene DeliveryDrug Delivery
Karaki T, Inoue W, Gis T, Shichinohe S, Watanabe T, Hirai T, Et Al.
Messenger RNA (mRNA) vaccines have emerged as versatile platforms for the prevention of infectious diseases and therapeutic cancer vaccination. However, strong reactogenicity remains a key challenge, with severe adverse events such as myocarditis. These concerns may limit the applications of mRNA vaccines. As lipid…
Read the article →ACS nano2026ResearchNon-viral Gene DeliveryDrug Delivery
Abbas Am, Ghanim R, Rudden S, Schultz D, Jang B, Shakked A, Et Al.
Lipid nanoparticles (LNPs) administered parenterally often show poor localization to multiple tissue types, including the pancreas and gastrointestinal (GI) tract. Furthermore, patients typically prefer orally administered drugs to those that are given parenterally. We therefore investigated whether GI LNP…
Read the article →ACS nano2026ResearchNon-viral Gene DeliveryDrug Delivery
He Y, Wang L, Williams Ap, Valerio Ti, Sun Y, Bowman J, Et Al.
In this study, we developed a library of mRNA lipid nanoparticles (LNPs) containing clinically used ionizable lipids. We surface-modified these nanoparticles with heparosan polysaccharides and systematically compared their performance with conventional poly(ethylene glycol) (PEG)-modified formulations. Our heparosan…
Read the article →ACS applied bio materials2026ResearchNon-viral Gene DeliveryDrug Delivery
Wang W, Liu H, Tang W, Shin J, Kim Yc, Li S
Lipid nanoparticles (LNPs) are widely explored as delivery vehicles for self-amplifying RNA (saRNA); however, incorporation of surface functionalities, such as fluorescent dyes for imaging and ligands for targeted delivery, remains challenging. In this study, we introduce a reactive poly(pentafluorophenyl acrylate)…
Read the article →Advanced materials (Deerfield Beach, Fla.)2026ResearchDrug Delivery
Knol Ra, Hu B, Bobylev Eo, Farber M, Laan Pcm, Kock Tjf, Et Al.
Inorganic anticancer drugs such as cisplatin remain central to chemotherapy but are limited by poor tumor selectivity, rapid clearance, and off-target toxicity. We recently developed cationic Pt 2 L 4 nanocages with 12-fold higher anticancer activity than cisplatin in PC-3M-Pro4 cells in vitro, but their positive…
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