Science advances2026ResearchNon-viral Gene DeliveryDrug Delivery
Xiao H, Li X, Cai K, Leng Y, Miao X, Yin T, Et Al.
Liver fibrosis is a severe complication of diverse chronic liver diseases characterized by a dysregulated hepatic microenvironment, and effective clinical therapeutic strategies for this condition remain lacking to date. Chimeric antigen receptor macrophages (CAR-Ms) hold considerable therapeutic potential for liver…
Read the article →Nano letters2026ResearchNon-viral Gene DeliveryDrug Delivery
Liu H, Yang B, Chen S, Li Y, Zhang Y, Huang Y, Et Al.
Highly efficient lung-selective mRNA delivery is pivotal for advancing mRNA-based targeted therapy. Here, we report bis(phosphoramidate diester)-based ionizable cationic lipids (bpLs) for lung-specific mRNA delivery via a distinct intercellular transport mechanism. A bpL library is synthesized by modular headgroups…
Read the article →Frontiers in immunology2026ResearchNon-viral Gene DeliveryDrug Delivery
Li J, Goncalves B, Melamed G, O'neill D, Cozzone R, Iuliucci S, Et Al.
Lentivirus (LV)-based manipulation of immune cells is a commonly used approach in cell therapies. However, the most frequently used Vesicular Stomatitis Virus Glycoprotein (VSV-G) pseudotyping approach is inefficient to support LV gene delivery of mouse immune cells due to the lack of an optimal entry receptor. Here…
Read the article →Acta pharmaceutica (Zagreb, Croatia)2026ResearchNon-viral Gene DeliveryDrug Delivery
Li N, Qu Z, Du D
The application of nanotechnology in drug delivery systems has attracted considerable scientific interest, particularly with respect to improving the therapeutic efficacy and safety profiles of pharmacological agents. This review critically examines nanotechnology-based drug delivery systems for the management of…
Read the article →ACS nano2026ResearchNon-viral Gene DeliveryDrug Delivery
He Y, Wang L, Williams Ap, Valerio Ti, Sun Y, Bowman J, Et Al.
In this study, we developed a library of mRNA lipid nanoparticles (LNPs) containing clinically used ionizable lipids. We surface-modified these nanoparticles with heparosan polysaccharides and systematically compared their performance with conventional poly(ethylene glycol) (PEG)-modified formulations. Our heparosan…
Read the article →ACS nano2026ResearchNon-viral Gene DeliveryDrug Delivery
Abbasi S, Bockley Km, Dohr J, Chen E, Carter Dm, Berlinicke Ca, Et Al.
Active targeting can improve the efficacy of mRNA therapeutics, but ligand-mediated targeting with lipid nanoparticles (LNPs) remains challenging. Here, we studied the impact of polyethylene glycol (PEG)-lipid composition and triantennary N-acetylgalactosamine (TriGalNAc)-modification on the hepatic delivery…
Read the article →bioRxiv : the preprint server for biology2026ResearchNon-viral Gene DeliveryDrug Delivery
Ansong-Ansongton Y, Adanho Csa, Goreke U, Lawanprasert A, Vysotskiy M, Tang Y, Et Al.
Hemoglobinopathies, including sickle cell disease (SCD) and thalassemia syndromes, affect millions of individuals worldwide who have limited access to curative therapies. Autologous hematopoietic stem cell transplant following ex vivo CRISPR editing of the BCL11A erythroid enhancer reactivates fetal hemoglobin (HbF)…
Read the article →Molecular therapy. Nucleic acids2026ResearchNon-viral Gene DeliveryDrug Delivery
Qi R, Chen H, Chen R, Bao H, Sun Y, Xu R, Et Al.
Targeted delivery and controlled expression of mRNA encapsulated by lipid nanoparticles (mRNA-LNPs) are critical for the development of safe and effective mRNA medicines. However, efficient post-delivery regulation of mRNA-LNP expression remains challenging, and conventional approaches largely rely on modifying the…
Read the article →Bioengineering & translational medicine2026ResearchNon-viral Gene DeliveryDrug Delivery
Wang S, Jing S, Zou S, Zhang Y, Ge M, Gao K, Et Al.
HER2 overexpression is a pivotal oncogenic alteration in NSCLC and is associated with poor prognosis. Existing HER2-targeted therapeutics, including antibody-drug conjugates, have limited anticancer efficacy and prominent systemic toxicity, whereas radiotherapy-mediated tumor immunosuppression further hinders…
Read the article →ACS applied bio materials2026ResearchNon-viral Gene DeliveryDrug Delivery
Wang W, Liu H, Tang W, Shin J, Kim Yc, Li S
Lipid nanoparticles (LNPs) are widely explored as delivery vehicles for self-amplifying RNA (saRNA); however, incorporation of surface functionalities, such as fluorescent dyes for imaging and ligands for targeted delivery, remains challenging. In this study, we introduce a reactive poly(pentafluorophenyl acrylate)…
Read the article →Journal for immunotherapy of cancer2026ResearchNon-viral Gene DeliveryDrug Delivery
Rehman Au, Khan Mn, Akhtar A, Qin Q, Zhang X, Asghar Ma, Et Al.
Interleukin-7 receptor (IL7R) signaling is critical for T-cell survival, homeostasis, and immune function, but the therapeutic potential of increasing IL7R expression through messenger RNA (mRNA) delivery remains poorly defined. We investigated whether IL7R mRNA therapy could enhance antitumor immunity and improve…
Read the article →Biomaterials science2026ResearchNon-viral Gene Delivery
Neri-Cruz Ce, Carta D, Gautrot Je
Rational design of polycationic gene delivery vectors requires understanding how polymer chemistry governs RNA complexation, cellular uptake, and intracellular release kinetics. Poly(dimethylaminoethyl methacrylate) brushes were found to display striking potential for the delivery of siRNA, with high knockdown…
Read the article →Nanotechnology2026ResearchDrug Delivery
Le Th, Phan Lmt
Extracellular vesicles (EVs), particularly stem cell-derived exosomes, serve as vital natural nanocarriers in regenerative medicine, immune modulation, and targeted drug delivery due to their innate ability to transport bioactive cargo across complex biological barriers. Despite this potential, the therapeutic…
Read the article →Advanced materials (Deerfield Beach, Fla.)2026ResearchDrug Delivery
Knol Ra, Hu B, Bobylev Eo, Farber M, Laan Pcm, Kock Tjf, Et Al.
Inorganic anticancer drugs such as cisplatin remain central to chemotherapy but are limited by poor tumor selectivity, rapid clearance, and off-target toxicity. We recently developed cationic Pt 2 L 4 nanocages with 12-fold higher anticancer activity than cisplatin in PC-3M-Pro4 cells in vitro, but their positive…
Read the article →Science translational medicine2026ResearchNon-viral Gene Delivery
Mccormack Nm, Lee-Diaz S, Reyes Tr, Hogarth Mw, Covington Jl, Bragg Ca, Et Al.
Limb-girdle muscular dystrophy type R2 (LGMDR2) is a genetic disorder characterized by progressive skeletal muscle weakness and degeneration. It is caused by mutations in the DYSF gene, which prevent dysferlin expression. Dysferlin protein is essential for muscle cell membrane repair. Gene therapy using…
Read the article →Frontiers in genome editing2026ResearchNon-viral Gene Delivery
Yu Z, Li J, Liu M, Qi Q, Duan Z, Yang Z, Et Al.
Prime editing (PE) enables precise genome modifications without double-strand breaks, yet its application in large mammals has been hindered by low efficiency and the technical bottlenecks of zygote microinjection. This study aimed to establish a scalable PE platform in sheep via the direct electroporation of a PE3…
Read the article →Molecular therapy. Nucleic acids2026ResearchNon-viral Gene DeliveryDrug Delivery
Ramamurthy A, Scimonelli G, Murphy Wl, Saha K
Human T cells modified with nucleic acids constitute a powerful and emerging therapeutic modality for cancer, autoimmune disorders, and aging-related diseases. However, delivering nucleic acids, such as mRNA encoding synthetic receptors, transcription factors, cytokines, or genome editors to T cells can be…
Read the article →Journal of controlled release : official journal of the Controlled Release Society2026ResearchNon-viral Gene DeliveryDrug Delivery
Jacobs W, De Zwaan K, Rutten L, Van Wessel T, Boltje Tj, Cano Lq, Et Al.
Non-viral nanocarriers for oligonucleotide delivery have enormous therapeutic potential, but the factors determining cell type-specific uptake remain incompletely understood. One major cell surface component that delivery vectors encounter is the glycocalyx. While membrane glycans are established interactors for many…
Read the article →Angewandte Chemie (International ed. in English)2026ResearchNon-viral Gene DeliveryDrug Delivery
Gan J, Wang Y, Li M, Cui S, Zhu L, Yu X, Et Al.
Lipid nanoparticles (LNPs) have emerged as a promising platform for gene/drug delivery in cancer therapy, however their nonspecific biodistribution and extensive hepatosplenic sequestration result in insufficient intratumoral accumulation, thereby undermining the therapeutic efficacy. Here, we establish a sequential…
Read the article →Journal of controlled release : official journal of the Controlled Release Society2026ResearchNon-viral Gene DeliveryDrug Delivery
Nasr Ss, Tabah Oe, Kumar S, Poore S, Duncan Ga
Pulmonary delivery of lipid nanoparticles (LNPs) remains an area of significant interest, given the broad range of genetic disorders that could be addressed through localized administration of therapeutic nucleic acids to the lung. In this study, we investigated how incorporation of the clinically used lung…
Read the article →Research Square (preprint)2026ResearchNon-viral Gene DeliveryDrug Delivery
Gond C, Verma A, Singh P, Singh D, Tiwari Ak
The process of delivering bioactive medication ingredients into a patient's body is known as drug delivery. To create drug delivery systems (DDs), a range of natural resources and synthetic materials derived from natural resources can be used as building blocks. These DDs include liposomal systems, which are composed…
Read the article →International journal of nanomedicine2026ResearchNon-viral Gene DeliveryDrug Delivery
Rodrigues Alves Mt, Da Silva Wn, Azevedo Gv, Carvalho Costa Pa, Silva Gh, Figueiredo Mm, Et Al.
Lipid nanoparticles (LNPs) have enabled the clinical translation of nucleic acid therapeutics, and their composition plays a critical role in organ targeting and route-specific delivery. Intranasal (IN) administration offers direct access to the lungs, while reducing systemic exposure. However, designing LNPs capable…
Read the article →Research Square (preprint)2026ResearchNon-viral Gene DeliveryDrug Delivery
Nguyen A, Jiang M, Müller Jt, Molbay M, Carneiro Sp, Matzek D, Et Al.
Asthma continues to pose a major clinical burden despite the availability of modern biologic therapies. In eosinophilic asthma, the transcription factor GATA3 is a key regulator of Th 2 differentiation and type 2 cytokine production and therefore represents an attractive target for siRNA-based intervention. We…
Read the article →ACS applied bio materials2026ResearchDrug Delivery
Kumar A, Patri Sv, Chilumula S, Arjunan P, Ayyanar V, Hanchate P, Et Al.
The clinical effectiveness of doxorubicin (DOX) in hepatocellular carcinoma (HCC) remains constrained by poor tumor selectivity and the emergence of multidrug resistance. In this work, we examine how the electronic nature of the C-5 substituent on uracil-based nucleoside lipids shapes the physicochemical and…
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